Liposomal Clodronate For Treating Autoimmune Hemolytic Anemia

By Roxanne Cruz

Liposomal Clodronate For Treating Autoimmune Hemolytic Anemia

Liposomal Clodronate is lately used in many medical researches, mainly as a treatment for autoimmune hemolytic anemia, also known as AIHA. Although some other methods proved to be useful as well, especially splenectomy and the use of corticosteroids, this method could be very useful for achieving good results in significantly shorter period of time.

Clodronate was successfully used for treating different osteolytic bone diseases. In various researches, it proved itself to be very useful in so called liposome mediated macrophage suicide technique. This method of depleting macrophages provides very good results in a very short time. This is especially important when such fast results are needed for successful treatment.

Clodronate itself cannot pass different cell membranes. When encapsulated within liposomes, they will surely be eagerly eaten by different macrophages. When the drug concentration reach the expected level within the macrophage cell, the result is the destruction of this cell. To be more precise, it is irreversibly damaged and dies by apoptosis.

Besides giving very fast and reliable results, this method has other qualities. The drug is completely non-toxic. It is developed for in vivo use, and once released from the destroyed macrophage cell, it will soon be removed by the kidneys. The drug has extremely short half life once in circulation. Quickly achieved results can be very useful, especially in combination with other therapies.

In order to be useful, liposomal clodronate has to reach the infected organs. It cannot cross capillary walls. Until now, this method proved to be useful for depleting macrophages in liver, lymph nodes, lung, joints and other organs, including testis and peritoneal cavity. For achieving expected results, the drug has to be adequately administered.

Although it is possible to deplete macrophages in vitro, the method is specifically developed for in vivo research. Clodronate released from dead macrophages has very short half-life and will be rapidly removed by the kidneys. In the culture medium, dependent on the composition of the medium, it cannot escape so easily, and it can be partially accumulated by the surrounding cells.

The required temperature for storing and keeping this suspension is 4 degrees of Celsius. It is very important to take a good care about this temperature, and to use the product within a few days. Before using the product, it should reach a room temperature slowly. It means you have to leave it there for some time, and to shake it well before injecting it. Other things to remember are not to freeze the product ever, and not to expose it to temperatures above 30 degrees of Celsius.

The quantity may vary, but recommended intravenous injection should be less than 0,1 ml for every 10 grams of weight. This number can be higher in case of intraperitoneal injection of the drug. Clodronate is stored within the liposomes, and the concentration depends on its solubility.

Liposomal clodronate method for making red blood cells eating macrophages destroy themselves proved to be very useful in different treatments. Quickly achieved results aren't permanent, they last for about one week. It is important to adequately administrate the drug, to use separate syringe for your test animals and to make sure to clean the skin before injecting the suspension. This way you will avoid different contamination. If you use the same syringe for all the animals, make sure to shake it each time.


Post a Comment